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Micro and Nano Biomedical |
For nanomedicine, MA-tek adopts appropriate sample preparation protocols (such as vacuum-dried, negative staining, cryo, liquid (K-kit), ultramicrotome, etc) for different types of samples such as virus, vaccine, and nanoformulated agents, etc. Comprehensive information of their physicochemical parameters can be obtained.
 Direct observation in liquid |
 With advanced sample preparation techniques |
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Liquid sample TEM (K-kit)：A microchip-based holder to contain and seal liquid sample inside for direct TEM observation
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PLGA：poly (Lactic-co-glycolic Acid)
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Resovist®：an iron oxide nanoparticle-based MRI contrast agent，(Ferucarbotran)
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MSNs: Mesoporous Silica Nanoparticles
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TEM Image Analysis |
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Nanoformulation agents | The first figure shows gold nanoparticles distribution in cells, and the second figure shows nanoparticle morphology.
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Vaccine | The first figure is the viral particle morphology, and the second figure is the adjuvant.
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Application |
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A Doxorubicin Encapsulated Liposomes |
The images of the liposomes enclosed with drug of Adoxorubincin. (Original condition)
Cryo-TEM |
UA Negative Stain |
After an ultrasonic process at 37C for 2 hours, the liposomes were fractured to release the drug. (Leakage condition)
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The size/size distribution of liposome was counted and measured by cryo-TEM images.
Counted # : original cond. by 403ea, leakage cond. by 225ea
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NOAAs of abrasives in CMP slurry |
K-kit can be used for characterizing the primary and secondary particles in undiluted CMP slurry.
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Dynamic observation of NOAAs in liquid |
Dynamic observation of silicate nanoparticles in water |
Dynamic observation of polystyrene beads under various media |
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Nanomaterials & Chemicals |
Carbon nanotubes (WMCNT) in Water |
Al2O3 nanoparticles in NMP solution |
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Pigment nanoparticles of printer ink |
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Biological specimens & Nanopharmaceuticals |
The nucleoid of E.coli |
Collagen bundles in liquid |
Extracellular vesicles of platelets |
Abraxane® drugs (paclitaxel) in saline |
AuroVist® gold nanoparticles in PBS |
Liposomes with negative staining |
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Gold nanoparticles (AuNP) in rat blood |
K-kits allow direct observation of nanoparticles in blood. Simply place K-kit channel in contact with whole blood sample without any pretreatment, serum will be loaded into the channel by capillary effect. ( Anal. Chem. 2012, 84: 6312 −6316 ) |
Image-based statistic analysis of Particle Concentration (K-kit vs. ICP-MS) |
Image-based statistic analysis of aggregation and agglomeration of AuNPs in blood |
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Micella in aqueous solution |
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K-kit can be used for characterizing micelle particles in liquid, by imaging the particle morphology, size and size distribution.
To evaluate the result of micelles in liquid by using K-kit (with liquid), compared with the conventional way by Cu grid (without liquid).
- Test sample：Micelles solution with particle sizes of around 30-50nm
- K-kit：Gap0.2um/ SiN100nm ones in wet mode
- TEM：Hitachi HT7700 @100KV
In K-kit (Magn. x 10K) |
On Cu grid (Magn. x 10K) |
There’re many single-micelles that with sizes of around 30nm spread uniformly and only a very few amounts of lumpy objects found in K-kit. By using K-kit, one may characterizes the micelle particles in liquid, without the drying effect to result in aggregates or agglomerates.
In K-kit (Magn. x 20K) |
On Cu grid (Magn. x 20K) |
On the Cu grid, most of the micelles aggregated due to drying effect and only a very few single-micelles remained sporadically.
The sizes of those single-micelles, which observed of around 10nm, on Cu grid were much smaller than the ones in K-kit, due to the structure collapsed by drying.
MA-tek follows the international regulatory guidance and international standards to provide professional and high quality services on nano biomedical characterization/analysis.